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February 21, 2021
Midterm – Essay
February 21, 2021

B- [3pts] A monoclonal antibody has been prepared against band 3, an integral membrane protein of th… Show more B- [3pts] A monoclonal antibody has been prepared against band 3, an integral membrane protein of the erythrocyte plasma membrane. The antibody recognizes an epitope on the carboxy terminus of band 3. Incubation of the two membrane vesicle preparations with this antibody following by staining with a fluorescently labeled secondary antibody generates data similar to that shown in the micrographs above. What can you deduce about the structure of band 3 from these data? The two vesicle fractions are either untreated (–) or are incubated with the protease trypsin (+). The membrane vesicles are then washed, the membranes solubilized and the proteins denatured and analyzed by SDS gel electrophoresis and Western blotting using polyclonal antibodies against glycophorin A, an erythrocyte plasma membrane protein. The following data are obtained: • Show less

 

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